They used computer technology that aims to mimic real-life movement so that when the figure moves, it appears to do so in three dimensions.
They have combined this with the kind of motion sensing and facial recognition devices used on video game consoles.
This allows the digital painting to tell when a viewer is looking directly at it. When no one is looking, the painting poses in profile as if lost in thought.
However, when the view looks directly at the digital Mona Lisa, it offers a slightly questioning smile.
If the algorithms feels the viewer is behaving in a way it does not like, however, the painting will darken and turn away.
Mr Aziosmanoff and his colleagues said this gives the painting emotions, moods and a personality thanks to the artificial intelligence technology used to drive it.
Writing on their website, they said: 'Emotions are what causes the immediate reactions of the character.
'They are manifestations of medium and intensive affects, usually caused by something that arises in the immediate environment.
'The mood is the general mood of the character, for a specified period - from a few tens of minutes to a few days.
Public react to interactive Mona Lisa at French exhibition