“'The Night Gwen Stacy Died:' The End of Innocence & the Birth of the Bronze Age

In the episode "Reaction", Peter (as Spider-Man) ends up saving both MJ and Gwen. She then decides to talk to Peter about Harry's condition, as he is taking the Globulin Green formula.

In the episode "Intervention", after a symbiote-controlled Peter blows her off, Mary Jane gets Gwen to admit to her her true feelings for Peter, and urges her to "step up".

This version of Gwen Stacy is much more sensible, compassionate and peaceful than all the other versions. Gwen is often seen as Peter's psychological support and is known by him (and possibly more, including her father) for her infamous "look" by which her glasses lower and she stares at the "victim." To date, this is the only version of Gwen who wears glasses. Her appearance and personality appears to be partially influenced by Debra Whitman's character.

In "Nature vs. Nurture", the finale of the first season, Eddie Brock becomes Venom, ties up and gags Gwen, and then suspends her from a large balloon during the Thanksgiving day parade, hoping that she will fall to her death, thus mentally torturing Peter. While Spider-Man battles Venom, the webs holding Gwen to the float eventually snap and she is sent hurtling to the street, but she is saved when Mary Jane Watson, Flash Thompson, Liz Allan, Randy Robertson, and her other classmates using the now-deflated balloon to catch her. In the final moments of the episode, Gwen kisses Peter for the first time after she and her father have Thanksgiving dinner with him and his Aunt May.